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Table 1 PCR primers for SFHR DNA and RNA analysis

From: In vitrocorrection of cystic fibrosis epithelial cell lines by small fragment homologous replacement (SFHR) technique

Primer Sequence Location
CF1B (S) 5'-CCTTCTCTGTGAACCTCTATCA-3' Intron 9
CF6 (A) 5'-CCACATATCACTATATGCATGC-3' Intron 10
CF17 (S) 5'-GAGGGATTTGGGGAATTATTTG-3' Intron 9
CF22 (A) 5'-CTTGCTAAAGAAATTCTTGCTC-3' Intron 11
ΔF1 (S) 5'-TCCTGGATTATGCCTGGCACC-3' Exon 10
ΔF2 (A) 5'-GGCATGCTTTGATGACGCTTC-3' Exon 10
  1. Primer sequences are reported from ref. [7]