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Table 1 PCR primers for SFHR DNA and RNA analysis

From: In vitrocorrection of cystic fibrosis epithelial cell lines by small fragment homologous replacement (SFHR) technique

Primer

Sequence

Location

CF1B (S)

5'-CCTTCTCTGTGAACCTCTATCA-3'

Intron 9

CF6 (A)

5'-CCACATATCACTATATGCATGC-3'

Intron 10

CF17 (S)

5'-GAGGGATTTGGGGAATTATTTG-3'

Intron 9

CF22 (A)

5'-CTTGCTAAAGAAATTCTTGCTC-3'

Intron 11

ΔF1 (S)

5'-TCCTGGATTATGCCTGGCACC-3'

Exon 10

ΔF2 (A)

5'-GGCATGCTTTGATGACGCTTC-3'

Exon 10

  1. Primer sequences are reported from ref. [7]
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