Figure 9

Transmission EM of CFBE41o^- cells transfected at a lipid/DNA ratio having no transfection activity (1+/1-). Cells were fixed at 5 h (A and B) and at 72 h (C and E) after the start of transfection. (A) and (B) show labelled complexes adhering to the cellular membrane; (B) is a higher magnification of (A). Arrows indicate sites at the plasma membrane that appear to be destabilised by the adhering complex. (C)-(E) show a labelled lysosome in a transfected cell; (E) is at high magnification. In (E) the lipid/DNA complexes, adhering to the lysosome membrane, appear to be free within the cytoplasm (arrowhead). ly: lysosome. Bars: (A), (C), (D), (E): 300 nm; (B): 100 nm.