Figure 3

Analysis of DNA from 16HBE14o^- transfected cells. (A) PCR analysis of wild-type (WT) and ΔF508 alleles as a function of lipid to DNA charge ratios in 16HBE14o^- cells transfected with the ΔF508 DNA fragment. Replacement was indicated by the presence of the ΔF-allele after transfection at lipid to DNA ratios ranging from 42+/1- to 1+/1- (lanes from 1 to 7). DNA from untrasfected cells was amplified in lane 8 as negative control; (B) Densitometric analysis revealed that the maximum correction efficiency is reached with a lipid/DNA ratio of 2.2+/1-. With this cell line no correction was observed at ratios of 42+/1-, 12+/1-, 6+/1-and 1+/1-.