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Table 1 Primer sets used for RT-PCR

From: A role for the collagen I/III and MMP-1/-13 genes in primary inguinal hernia?

mRNA template Primer sequence (sense/anti-sense) PCR product size (bp) Annealing temperature
type I collagen 5'CCCCCTCCCCAGCCACAAAG-3' 5'-TCTTGGTCGGTGGGTGACTCT-3' 360 44°C
type III collagen 5'-CCAAACTCTATCTGAA-3' 5'-GGACTCATAGAATACA-3' 449 44°C
MMP-1 5'-AGGTTATCCCAAAATGATAG-3' 5'-TGCAGTTGAACCAGCTATTA-3' 173 55°C
MMP-13 5'-TTCTGGCACATGCTTTTCCTC-3' 5'-GGTTGGGGTCTTCATCTCCTG-3' 273 55°C
β-actin 5'-ATCTGGCACCACACTTCTACA-3' 5'-GTTTCGTGGATGCCACAGGACT-3' 577 55°C