From: A role for the collagen I/III and MMP-1/-13 genes in primary inguinal hernia?
mRNA template | Primer sequence (sense/anti-sense) | PCR product size (bp) | Annealing temperature |
---|---|---|---|
type I collagen | 5'CCCCCTCCCCAGCCACAAAG-3' 5'-TCTTGGTCGGTGGGTGACTCT-3' | 360 | 44°C |
type III collagen | 5'-CCAAACTCTATCTGAA-3' 5'-GGACTCATAGAATACA-3' | 449 | 44°C |
MMP-1 | 5'-AGGTTATCCCAAAATGATAG-3' 5'-TGCAGTTGAACCAGCTATTA-3' | 173 | 55°C |
MMP-13 | 5'-TTCTGGCACATGCTTTTCCTC-3' 5'-GGTTGGGGTCTTCATCTCCTG-3' | 273 | 55°C |
β-actin | 5'-ATCTGGCACCACACTTCTACA-3' 5'-GTTTCGTGGATGCCACAGGACT-3' | 577 | 55°C |