Figure 2

Immunoblots of total cell lysates from wild-type (WT) and mutant (M) fibroblast clones and lymphoblastoid cell lines used for the oligonucleotide expression array hybridization studies. MeCP2 expression is detected with a C-terminal antibody; the molecular weight of MeCP2 is shown as 75 kDa, consistent with the company's information http://www.upstate.com. In mutant cell lines with nonsense mutations (FB 705delG, FB 1155del32, and LCL R168X), an intact MECP2 protein is not seen. Brain lysate from mice and normal human male lymphoblastoid cells serve as positive controls.