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Table 1 Primer sequences used to amplify and sequence all the coding exons of the TBX1 and CRKL gene and exon/intron boundaries

From: Novel TBX1loss-of-function mutation causes isolated conotruncal heart defects in Chinese patients without 22q11.2 deletion

Primers Orientation Sequences Fragment size
TBX1-EXON1 F 5’-AGGAGCAGATGTCTCAGCCC-3’ 594 bp
R 5’-CACCGGCTGCCTATACTCAC-3’
TBX1-EXON2 F 5’-ATGACGCCATAATCCTCTGG-3’ 725 bp
R 5’-TGTGTTTTCTCCCCTTTGCT-3’
TBX1-EXON3 F 5’-TCACGCAGCTCTCGCATTTC-3’ 628 bp
R 5’-CCGGCGGAGGATAGGTGTTA-3’
TBX1-EXON4 F 5’-CAAGCTCCCAGTTGAGTAGG-3’ 414 bp
R 5’-GCAGGTGCCTAAAGAGTTTC-3’
TBX1-EXON5 F 5’-GGCAGCAGAGGGTTCAATCT-3’ 457 bp
R 5’-GCCTCGCAGGGACTCTAAAG-3’
TBX1-EXON6 F 5’-TGACCCAGCCTCATCTTGGA-3’ 404 bp
R 5’-AGGTCTAAGCGGACCCACTG-3’
TBX1-EXON7-8 F 5’-GGTGCGCTTCTCCTAACACTC-3’ 722 bp
R 5’-GGAGAGGGCCGAGGAGTG-3’
TBX1-EXON9C F 5’-CCAAGAGCCTTCTCTCCGC-3’ 775 bp
R 5’-TGGGGAACCGGATACTTCGA-3’
TBX1-EXON9A F 5’-CGTTGGGAGATGCAGTCCT-3’ 853 bp
R 5’-GCTTACTGGACAGCAGCAC-3’
TBX1-EXON9B F 5’-CTGATGGTGTGTGAGGCTGA-3’ 636 bp
R 5’-CACCTCTTGCATGCACACTT-3’
TBX1-EXON10 F 5’-CTGCTCTGTTTGAGGTGGTT-3’ 632 bp
R 5’-ACGCATCAGCTTTTATGGGA-3’
CRKL-EXON1_1 F 5’- GACGGTGCTCCTGATTGGCT-3’ 747 bp
R 5’-CCCAGGGCAGGTGGAAGAAT-3’
CRKL-EXON1_2 F 5’-GGACAGCCGCCGCCCCTACC-3’ 434 bp
R 5’-CCCACCCCCCTATACAAGCA-3’
CRKL-EXON2 F 5’-CAGTGAGCTAAGATCACGCT-3’ 677 bp
R 5’-ATGTCAAAGGACCCAAAAAG-3’
CRKL-EXON3 F 5’-AAGGATCACTTGAGCCCAGG-3’ 376 bp
  R 5’-AGGCAGAACAACAAAGCAGC-3’  
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BMC Medical Genetics

ISSN: 1471-2350

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