Table 1 Primer sequences used to amplify and sequence all the coding exons of the TBX1 and CRKL gene and exon/intron boundaries
Primers | Orientation | Sequences | Fragment size |
|---|---|---|---|
TBX1-EXON1 | F | 5’-AGGAGCAGATGTCTCAGCCC-3’ | 594 bp |
R | 5’-CACCGGCTGCCTATACTCAC-3’ | ||
TBX1-EXON2 | F | 5’-ATGACGCCATAATCCTCTGG-3’ | 725 bp |
R | 5’-TGTGTTTTCTCCCCTTTGCT-3’ | ||
TBX1-EXON3 | F | 5’-TCACGCAGCTCTCGCATTTC-3’ | 628 bp |
R | 5’-CCGGCGGAGGATAGGTGTTA-3’ | ||
TBX1-EXON4 | F | 5’-CAAGCTCCCAGTTGAGTAGG-3’ | 414 bp |
R | 5’-GCAGGTGCCTAAAGAGTTTC-3’ | ||
TBX1-EXON5 | F | 5’-GGCAGCAGAGGGTTCAATCT-3’ | 457 bp |
R | 5’-GCCTCGCAGGGACTCTAAAG-3’ | ||
TBX1-EXON6 | F | 5’-TGACCCAGCCTCATCTTGGA-3’ | 404 bp |
R | 5’-AGGTCTAAGCGGACCCACTG-3’ | ||
TBX1-EXON7-8 | F | 5’-GGTGCGCTTCTCCTAACACTC-3’ | 722 bp |
R | 5’-GGAGAGGGCCGAGGAGTG-3’ | ||
TBX1-EXON9C | F | 5’-CCAAGAGCCTTCTCTCCGC-3’ | 775 bp |
R | 5’-TGGGGAACCGGATACTTCGA-3’ | ||
TBX1-EXON9A | F | 5’-CGTTGGGAGATGCAGTCCT-3’ | 853 bp |
R | 5’-GCTTACTGGACAGCAGCAC-3’ | ||
TBX1-EXON9B | F | 5’-CTGATGGTGTGTGAGGCTGA-3’ | 636 bp |
R | 5’-CACCTCTTGCATGCACACTT-3’ | ||
TBX1-EXON10 | F | 5’-CTGCTCTGTTTGAGGTGGTT-3’ | 632 bp |
R | 5’-ACGCATCAGCTTTTATGGGA-3’ | ||
CRKL-EXON1_1 | F | 5’- GACGGTGCTCCTGATTGGCT-3’ | 747 bp |
R | 5’-CCCAGGGCAGGTGGAAGAAT-3’ | ||
CRKL-EXON1_2 | F | 5’-GGACAGCCGCCGCCCCTACC-3’ | 434 bp |
R | 5’-CCCACCCCCCTATACAAGCA-3’ | ||
CRKL-EXON2 | F | 5’-CAGTGAGCTAAGATCACGCT-3’ | 677 bp |
R | 5’-ATGTCAAAGGACCCAAAAAG-3’ | ||
CRKL-EXON3 | F | 5’-AAGGATCACTTGAGCCCAGG-3’ | 376 bp |
R | 5’-AGGCAGAACAACAAAGCAGC-3’ |