The phylogenetic sequence alignment of the T-box domain and 20 additional amino acids from different species located at the N and C terminus of the T-box domain. The phylogenetic analysis is from vertebrate mammals to marine zooplankton. The aminoacid residues that are identical to the TBX1 sequences are shaded. The red pentagram demonstrates the amino acid substitutions of E129K and G310S in the TBX1 found in patients. The dotted line indicated the T-box domain.