Table 2 Sequence and location of PCR and sequencing primers
PCR name | Region | Primers sequence (forward and reverse) | Location of primers | PCR product size | PCR annealing temperature (°C) |
|---|---|---|---|---|---|
CYP3A4 gene | |||||
A | 5′ Proximal Region | A1: GGTCTGTCTGTCTGGGTATGC | 296 | 61 | |
A2: CTCACCACACACTGACCTGCT | |||||
B | EXON 1 (nt 1–71) | B1: AGAACCCAGAACCCTTTGGAC | 1 | 1137 | 59 |
B2: GTGCTCCTCTATCTGTGAGTA | 78 | ||||
C | EXON 2 (nt 4004–4097) | C1: GCTCTCAGTGACCCTCTGTG | 3532 | 1192 | 59 |
C2: AACCCCTTTGTTCTGTCTCTCA | 4723 | ||||
D | EXON 3 (nt 6009–6061) | D1: CCCTGGTGTCTGTACTTTCCA | 5529 | 1200 | 59 |
D2: TCCCAGCCTAGTTCAGACTGT | 6728 | ||||
E | EXON 4 (nt 11502–11601) | E1: ATATCCACGTATGCACCACCC | 11185 | 841 | 59 |
E2: GAGCCACATGGAGACAGAGT | 12025 | ||||
F | EXONS 5/6 (nt 13956–14069) (nt 14335−14423) | F1: CGACATCAGGGTCTCCTGAAC | 13720 | 933 | 59 |
F2: GATATGTAAACCCTGGCCCCT | 14652 | ||||
G | EXON 7 (nt 15689–15837) | G1: CTGTTTGTCTGTCTTGACTGGA | 15585 | 998 | 61 |
G2: GCTGTTCAAGAAATAGTAGGTAGTC | 16582 | ||||
H | EXON 8 (nt 16932–17059) | H1: TTGAGCTTCAGATTATGATTTGGG | 16593 | 956 | 60 |
H2: CTGGCTATCATGTGAGATGGC | 17548 | ||||
I | EXON 9 (nt 17744–17810) | I1: AGCCATCTCACATGATAGCCA | 17527 | 990 | 57 |
I2: CTTGGTGGCTTGTAATTGACC | 18516 | ||||
J | EXON 10 (nt 20166–20326) | J1: TGGGGGAGAGTACTACCTCATA | 19785 | 952 | 60 |
J2: AAGAGCCAATTCCTGTGTCCAT | 20736 | ||||
K | EXON11 (nt 21912–22138) | K1: TTCCCGAATGCTTCCCACCT | 21691 | 917 | 59 |
K2: ATGCTACTGTACCGATGTAATGC | 22607 | ||||
L | EXON 12 (nt 23198–23360) | L1: GGGGTGGCCCCTAAGTAAGA | 23109 | 910 | 57 |
L2: TTGGGTTGAAAAGGAGCCCA | 24018 | ||||
M | EXON 13 (nt 25950–26502) | M1: TGACTCTTCAAAAACAGTTTGCCA | 25518 | 1099 | 59 |
M2: AGTTCTGACAAAGGCCCCAC | 26502 | ||||
CYP3A5 gene | |||||
N | EXON 1 (5001–5173) | N1: TAGAATGAAGGCAGCCATGGAG | 4723 | 1032 | 60 |
N2: GGGGATTTTCAGGGGCATGG | 5774 | ||||
O | EXON 2 (8791–8884) | O1: GCTGGTTCTTCTGCACACAATC | 8022 | 964 | 61 |
O2: GAAACCTCAGAACTCCCTCCC | 8985 | ||||
P | EXON 3 (10414–10466) | P1: ATGGAGAGTGGCATAGGAGAT | 9878 | 1177 | 59 |
P2: TGTGGTCCAAACAGGGAAGAGAT | 11054 | ||||
Q | EXON 4 (12320–12419) | Q1: TGTCACCAGGTATCGAGGTCT | 11062 | 1367 | 60 |
Q2: GATGCTTACCCTTCGATTTGTGA | 12428 | ||||
R | EXONS 5/6 (17934–18047) (18310–18398) | R1: CGCCCCACATACACTCAGAA | 17746 | 1184 | 57 |
R2: GGCTTGCTCTACACATAGCAT | 18929 | ||||
S | EXON 7 (19685–19833) | S1: ATAGGGCCAGCTCCATCACTG | 19492 | 1193 | 60 |
S2: TTCTGAGTCTTTGGAGTGACCA | 20684 | ||||
T | EXONS 8/9 (20904–21031) (22117–22183) | T1: GGCCTGAAAGAAGGGCAAAC | 20750 | 1478 | 57 |
T2: TCTTAGTGTCCCCGCCAGTA | 22228 | ||||
U | EXON 10 (24340–24500) | U1: AGGATCATTCAAGGCACACACC | 24009 | 1179 | 61 |
U2: GCCTTGCTGCTGCCTTGCAG | 25188 | ||||
V | EXON 11 (32220–32446) | V1: ACCTACCTATGATGCCGTGG | 32225 | 880 | 57 |
V2: GAGGACCTGTGCTGTCTTGT | 33104 | ||||
W | EXON 12 (34767–34926) | W1: GCAGGATTTCAATGACCAGCC | 34619 | 1173 | 59 |
W2: CCCCCTGCCTGAATACACAC | 35792 | ||||
Y | EXON 13 (36599–36809) | Y1: GGGTTCAACTGGGAAGGGTT | 36118 | 1058 | 57 |
Y2: GTGTGCAGGATGGCATCAGA | 37175 |