Table 2 Primer sequences used in RT-PCR and real-time quantitative RT-PCR
From: Five novel mutations in the ADAR1 gene associated with dyschromatosis symmetrica hereditaria
RT-PCR | E1F: 5′-GAGGAAACGAAAGCGAAATTGAACCGGA-3′ |
E2F: 5′- AACTCCACATCTGCCTTGGAAGATCCTC -3′ | |
E3R: 5′-CTCTCGGCCATTGATGACAACCTGGAAT-3′ | |
E4R: 5′-TGTGCATACACTCAAGCAGTGTGGTGAC-3′ | |
Real-time RT-PCR | E9-10 F: 5′-GGCTTCATCAGGTTTCTCTAC-3′ |
E10-11R: 5′-CACGGAGCAGTGCTGATATAC-3′ | |
GAPDH-F: 5′-CATCTTCCAGGAGCGAGATC-3′ | |
GAPDH-R: 5′-GCAAATGAGCCCCAGCCTTC-3′ |