Table 1 Methylation by SGA status
MLPA | SGA (N = 80) % mean methylation (standard deviation) | AGA (N = 72) % mean methylation (standard deviation) | T-statistic (p value) |
|---|---|---|---|
H19 ICR (1) | 56.3 (7.3) | 57.3 (7.6) | −0.84 (0.40) |
H19 ICR (2) | 62.0 (6.6) | 61.9 (5.7) | 0.11 (0.92) |
H19 ICR (3) | 62.7 (6.1) | 61.9 (6.6) | 0.75 (0.45) |
H19 ICR (4) | 68.2 (9.1) | 67.3 (7.5) | 0.69 (0.49) |
H19 ICR (5) | 87.2 (7.7) | 85.8 (6.7) | 1.10 (0.27) |
Average | 66.4 (4.7) | 66.3 (3.7) | 0.17 (0.87) |
KCNQ10T1 (1) | 58.3 (7.5) | 60.0 (7.5) | −1.41 (0.16) |
KCNQ10T1 (2) | 54.1 (6.7) | 53.9 (6.9) | 0.16 (0.87) |
KCNQ10T1 (3) | 54.7 (7.6) | 55.9 (7.1) | −0.99 (0.33) |
KCNQ10T1 (4) | 61.7 (9.1) | 63.2 (8.9) | −1.01 (0.31) |
KCNQ10T1 (5) | 66.7 (11.0) | 67.4 (14.6) | −0.32 (0.75)* |
Average | 57.2 (5.4) | 58.2 (5.6) | −1.17 (0.24) |
Pyrosequencing (CTCF3 H19 DMR) | SGA (N = 43) | AGA (N = 15) | |
CpG2 | 41.6 (3.1) | 40.6 (3.1) | 1.03 (0.31) |
CpG3 | 50.4 (7.4) | 49.9 (11.1) | 0.17 (0.87)* |
CpG4 | 60.1 (5.1) | 61.6 (10.3) | −0.56 (0.58)* |
CpG5 | 40.6 (3.3) | 40.1 (3.1) | 0.48 (0.63) |
CpG6 | 46.2 (2.7) | 45.5 (3.0) | 0.82 (0.42) |
CpG7 | 45.1 (2.2) | 45.2 (2.5) | −0.10 (0.92) |
CpG8 | 44.6 (2.4) | 44.3 (2.9) | 0.41 (0.68) |
CpG9 | 71.4 (6.5) | 71.4 (9.6) | 0.01 (0.99) |
CpG10 | 43.3 (3.7) | 41.8 (2.9) | 1.42 (0.16) |
CpG11 | 42.6 (2.6) | 42.0 (2.5) | 0.82 (0.41) |
CpG12 | 38.5 (2.5) | 38.2 (3.2) | 0.38 (0.71) |
Average | 47.3 (2.8) | 47.3 (3.4) | −0.05 (0.96) |
IGF2DMR0 | |||
CpG1 | 44.1 (3.8) | 47.2 (3.8) | −2.69 (0.0094) |
CpG2 | 55.3 (2.8) | 56.9 (4.3) | −1.37 (0.19) |
CpG3 | 61.7 (3.7) | 64.5 (10.2) | −1.04 (0.31)* |
Average | 53.6 (2.5) | 56.2 (5.1) | −1.90 (0.075) |