Molecular basis of DEL phenotype in the Chinese population

Gu, Juan; Wang, Xue-Dong; Shao, Chao-Peng; Wang, Jun; Sun, An-Yuan; Huang, Li-Hua; Pan, Zhao-Lin

doi:10.1186/1471-2350-15-54

BMC Medical Genetics

Table 1 Primers used for PCR analysis and DNA sequencing

From: Molecular basis of DEL phenotype in the Chinese population

Primer name*	Sequence 5′ to 3′	Location^#	Specificity	Product size (bp)
E1-s	ATGCCTGGTGCTGGTGGA	promoter, -43 to -26	RHD/CE	228
E1-a	ATTTGCTCCTGTGACCACTT	intron 1, 37 to 18	RHD	228
E1-seq	ATGCCTGGTGCTGGTGGA
E2-s	TGACGAGTGAAACTCTATCTCGAT	intron 1, -1064 to -1041	RHD	1475
E2-a	GGATTCCTTGTGATACACGGAGTAG	intron 2, 224 to 200	RHD	1475
E2-seq	TGACGAGTGAAACTCTATCTCGAT
E3-s	GTCGTCCTGGCTCTCCCTCTCT	intron 2, -29 to -8	RHD	219
E3-a	CTTTTCTCCCAGGTCCCTCCT	intron 3, 39 to 19	RHD/CE	219
E3-seq	GTCGTCCTGGCTCTCCCTCTCT
E4-s	GCCGACACTCACTGCTCTTAC	intron 3, -36 to -16	RHD/CE	378
E4-a	TGAACCTGCTCTGTGAAGTGC	intron 4, 194 to 174	RHD	378
E4-seq	GCCGACACTCACTGCTCTTAC
E5-s	TACCTTTGAATTAAGCACTTCACAG	intron 4, -267 to -233	RHD	1458
E5-a	TTATTGGCTACTTGGTGCC	intron 5, 1024 to 1006	RHD	1458
E5-seq	TACCTTTGAATTAAGCACTTCACAG
E6-s	CAGGGTTGCCTTGTTCCCA	intron 5, -95 to -77	RHD/CE	274
E6-a	CTTCAGCCAAAGCAGAGGAGG	intron 6, 41 to 21	RHD	274
E6-seq	CAGGGTTGCCTTGTTCCCA
E7-s	TGCCCATCCCCCTTTGGTGGCC	intron 6, -106 to -85	RHD	411
E7-a	CCAAGGTAGGGGCTGGACAG	intron 7, 171 to 152	RHD	411
E7-seq	TGCCCATCCCCCTTTGGTGGCC
E8-s	GGTCAGGAGTTCGAGATCAC	intron 7, -594 to -575	RHD	709
E8-a	TGGCAATGGTGGAAGAAAG	intron 8, 35 to 16	RHD/CE	709
E8-seq	GGTCAGGAGTTCGAGATCAC
E9-s	CTGTCGTTTTGACACACAATATTTC	intron 8, -91 to -67	RHD	190
E9-a	CACGTTAATAGGTGAAAAATCTTACC	intron 9, 25 to exon 9, 1227	RHD	190
E9-seq	CTGTCGTTTTGACACACAATATTTC
E10-s	CAAGAGATCAAGCCAAAATCAGT	intron 9, -67 to -45	RHD/CE	382
E10-a	AGCTTACTGGATGACCACCA	3’UTR, 291 to 272	RHD	382
E10-seq	CAAGAGATCAAGCCAAAATCAGT
RHD1227A-s	GATGACCAAGTTTTCTGGAAA	exon 9, 1207 to 1227	RHD1227A	109
RHD1227A-a	CATAAACAGCAAGTCAACATATATACT	intron9, 88 to 62	RHD	109
RHD1227A-seq	GATGACCAAGTTTTCTGGAAA
β-actin-s	GGAAATCGTGCGTGACATT	—		473
β-actin-a	CGTCATACTCCTGCTTGCTG	—		473

*s = sense primer; a = antisense primer. seq = sequencing primer.
^#The positions of the synthetic oligonucleotides are indicated relative to their distances from the first nucleotide position of the start codon ATG for all primers in the promoter and in the exons or relative to their adjacent exon-intron boundaries for all other primers.

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ISSN: 1471-2350

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