Figure 1

Results of the PCR-SSP analysis. In all lanes, a 473-bp product was amplified as the internal positive control; M, molecular marker (2000, 1000, 750, 500, 250 and 100 bp, respectively). (a) PCR-SSP for RHD1227A allele in 9 DEL samples. Lanes 1 to 9 showed the PCR-SSP results of 9 DEL samples. Lanes 1 to 5 showed that the RHD1227A specific amplifications (band of 109 bp) were positive (Sample No.13001-13005). They were shown as representatives of 37 DEL individuals carrying RHD1227A allele. Lane 6 to 9 showed that the RHD1227A specific amplifications were negative (Sample No.13038-13041). (b) PCR-SSP for RHD-CE (4–7)-D in one DEL sample (Sample No.13039). Lanes 1 to 10 showed the PCR-SSP results of the RHD exons 1 to 10; Lanes 4 to 7 showed that the RHD specific amplifications were negative for exons 4 to 7; RHD specific amplifications were positive for exons 1 to 3 and 8 to 10. (c) PCR-SSP for RHD-CE (2–5)-D in one DEL sample (Sample No.13038); lanes 1 to 10 showed the PCR-SSP results of the RHD exons 1 to 10; Lanes 2 to 5 showed that the RHD specific amplifications were negative for exons 2 to 5; RHD specific amplifications were positive for exons 1, 6 to 10.