Comparison of intracellular localization of DDR2 wild type and S823Cfs* mutant in relation with plasma membrane marker H-Ras and ER marker calnexin. HeLa cells were co-transfected with C-terminally HA-tagged wild type or mutant DDR2 and EGFP tagged H-Ras constructs and stained with anti-HA antibodies. (A) and (D) shows the localization patterns of WT and the mutant DDR2 proteins. (B) and (E) shows the localization of the marker protein H-Ras to the plasma membrane. (C) shows co-localization of WT-DDR2 with H-Ras and (F) shows exclusion of S823Cfs*-DDR2 from the plasma membrane. (G) and (J) show distribution of HA-tagged wild type DDR2 and S823cfs* mutant in HeLa cells transfected with the indicated construct. (H) and (K) show the localization of the ER marker protein calnexin. (I) and (L) show the extent of co-localization of DDR2 proteins with calnexin. For presentation purpose, images (G)-(L) were pseudocolored as either red (DDR2) or green (calnexin) using ImageJ software.