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Figure 5 | BMC Medical Genetics

Figure 5

From: A simple method for gene phasing using mate pair sequencing

Figure 5

One phase of the entire 10 kb amplicon. By beginning with the first heterozygote in the amplicon and sequentially moving through all downstream heterozygous positions, the phase of the entire 10 kb amplicon can be determined. Confidence intervals in the columns show the relationship of each base to the highest probability base call from the previous column. Lines showing the cumulative probability and confidence interval relate each downstream position to the very first in the chain. Cumulative probability diminishes in proportion to the quality of each association matrix in the chain (i.e. sufficient coverage and few errors). However, one can be sure of the accuracy of phasing so long as there is no overlap between the cumulative confidence interval and that of any rejected base.

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