Evaluation of DNA damage in COPD patients and its correlation with polymorphisms in repair genes

BMC Medical Genetics

Table 1 Amplification conditions, restriction enzymes annealing temperature and fragment size of the studied genes

Gene	Primer sequence	SNP	AT (°C)	Restriction enzyme	Fragment sizes (bp)	Reference
OGG1	3'GTGGATTCTCATCGGTTCG 5'	Ser326Cys	58	Fnu4HI	672	Ruyck et al. (2005) [28]
OGG1	5'CTGTTGCTGTCGAGAATGC 3'	Ser326Cys	58	Fnu4HI	672	Ruyck et al. (2005) [28]
XRCC1	3'CAAGTACAGCCAGGTCCTAG 5'	Arg399Gln	58	BcnI	268	Chiyomaru et al. (2012) [29]
XRCC1	5'CCTTCCCTCATCTGGAGTAC 3'	Arg399Gln	58	BcnI	268	Chiyomaru et al. (2012) [29]
XRCC3	3'GCCTGGTGGTCATCGACTC 5'	Thr241Met	67	NcoI	552	Krupa et al. (2009) [30]
XRCC3	5'ACAGGGCTCTGGACAGCTCACGTCAC 3'	Thr241Met	67	NcoI	552	Krupa et al. (2009) [30]
XRCC4	3'CTCAGAAGAAATTGTGTATGCT 5'	Ile401Thr	52	BseMI	277	Relton et al. (2004) [31]
	5'ACCACAAGCAAACTGTGTACAC 3'

SNP, single-nucleotide polymorphism; AT, annealing temperature in degrees Celsius; bp, bases pairs.

ISSN: 1471-2350