Figure 4

Cell migration assay of proband and control fibroblasts. Live cell imaging was used to monitor movement of cells into an open scratch wound over 72 h, with images being captured every 1 h. A: Image snapshots were captured at 0, 36 and 72 h after post-wound image capture began. Live cells have been stained with Cell Tracker^™ Blue fluorescent dye, and a white line marks the edge of the scratch wound. Scale bar represents 200 μm. B: Quantitative representation of distance covered by cells within the scratch wound over the 72-h analysis period. Results are expressed as mean ± SEM % distance covered by the cells per unit time (h), with 100% representing complete filling of the wound; n = 3 scratches for each cell type.