High Resolution Melting analysis as a rapid and efficient method of screening for small mutations in the STK11gene in patients with Peutz-Jeghers syndrome

Table 1 List of primers utilised in the HRM analysis

Amplicon	Forward primer	Reverse primer	Product length [bp]	Annealing temperature [°C]
1a	GGAAGTCGGAACACAAGGAA	CATCAGGTACTTGCCGATGA	231	60
1b	CTCCACCGAGGTCATCTACC	CCCCAGCAAGCCATACTTAC	221	60
2	AGGCCATCATCCTGACGTT	CCCAACACCGGAAAGGATA	159	60
3	CTCCCTGGGCCTGTGAGT	CCCTGCCCCGCGCACGCA	165	60
4a	CAGGACGGGTGTGTGCTG	CCCTAGCACGTGCCTACCT	175	60
4b	GCCTGGAGTACCTGCATAGC	GTGCAGCCCTCAGGGAGT	172	60
5a	CACTCCCTGAGGGCTGCAC	GGGGCACTTACAGGGTGAC	180	60
5b	GGCTTCAAGGTGGACATCTG	CTGTGGCCAGAGAGGGTCT	161	60
6	TCAACCACCTTGACTGACCA	ACCCCCAACCCTACATTTCT	249	60
7	CCTTAGGAGCGTCCAGGTATC	CTCAACCAGCTGCCCACAT	244	60
8a	CTGGGTCGGAAAACTGGAC	GTGCAGGTCCTCCAAGTACG	182	60
8b	GAGCCCAGACACCAAGGAC	GACATCCTGGCCGAGTCA	188	60
9	TCAGCTCAGGCCACACTTGC	AGCCTCACTGCTGCTTGC	232	60

ISSN: 1471-2350