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Table 2 Primer sequences used for nested real-time PCR

From: Pathogenic substitution of IVS15 + 5G > A in SLC26A4 in patients of Okinawa Islands with enlarged vestibular aqueduct syndrome or Pendred syndrome

Nested PCR assay

Sequence

PCR product size (bp)

First-step PCR (external primer)

Exon 14

forward

TCTTGGAATGGCCTTGGAAGC

282

Exon 17

reverse

TGAAACAGCATCACTTATGATGC

Second-step PCR (internal primer)

Exon 15

forward

TGAAGAACCTCAAGGAGTGAAG

154

Exon 16

reverse

TTTCTGTATTTTCCTCAGCGCT