Nested PCR assay | Sequence | PCR product size (bp) | ||
---|---|---|---|---|
First-step PCR (external primer) | Exon 14 | forward | TCTTGGAATGGCCTTGGAAGC | 282 |
Exon 17 | reverse | TGAAACAGCATCACTTATGATGC | ||
Second-step PCR (internal primer) | Exon 15 | forward | TGAAGAACCTCAAGGAGTGAAG | 154 |
Exon 16 | reverse | TTTCTGTATTTTCCTCAGCGCT |