Bilateral radial agenesis with absent thumbs, complex heart defect, short stature, and facial dysmorphism in a patient with pure distal microduplication of 5q35.2-5q35.3

Table 1 Sequences of the primers used for TBX5 **gene (MIM ID*601620; GenBank NM_000192) amplification and sequencing**

Exon name (fragment)	Forward primer sequence 5^′- 3^′	Reverse primer sequence 5^′- 3^′	Product size (bp)
TBX5_e2	TCTCTCTCTGTCCTCCCCAC	CAAGAGAAGCCGAGCAGG	310
TBX5_e3	GGGAAGGAATGCCCACTAC	TTCCaagccaccttttcttc	231
TBX5_e4	TTAAAATGGATGGAGGCTGC	TTTTGGGAGAAGGTTCCAC	256
TBX5_e5	GTGCAGTGCGCTACCTCC	GAAACCCagtgagaagaaggg	279
TBX5_e6	GGTTTTATCTGGAGACAAAGGG	CAGGAAAACCTTGCAGATTC	293
TBX5_e7	CATGTCCTGAGGTGGTCTTG	GTTGCTGCTGGCTTACCTG	232
TBX5_e8	TCAGCCACTCAGGAAATCTG	CCCCAACCCAAGGAAAGG	356
TBX5_e9	CTCCACTTTTAGCTGCCTGG	TAGATCAGCATCCAGCGACC	797

ISSN: 1471-2350