Genetic mapping of high caries experience on human chromosome 13

BMC Medical Genetics

Table 3 Primers used for mutation search and gene expression analyses

Primers used for mutation search
Primer	Primer sequences (5′–3′)		Amplicon size (base pairs)	Thermal cycling condition
Forward	CAGCTTTATCGCCAGAGTCC		350	94°C 5 min→ 35×[94°C 30 sec, 56°C 30 sec, 72°C 30 sec]→72°C 5 min
Reverse	CCTCTTCCTCACCATCACCT		350
Forward	AAGAAGGTGGGGAGGAAGAG		550	94°C 5 min→ 35×[94°C 30 sec, 53°C 30 sec, 72°C 30 sec]→72°C 5 min
Reverse	GGTCATCCGAGATCATTAAAAA		550
Forward	TTTTTATACATATTGTTAGGGTCAGC		500	94°C 5 min→ 35×[94°C 30 sec, 53°C 30 sec, 72°C 30 sec]→72°C 5 min
Reverse	CAGTTTGTTTACCATCTCAAACACTT		500
Primers used for quantitative real-time polymerase chain reaction analysis
Target Gene	Primer	Primer sequences (5′–3′)	Amplicon size	Thermal cycling condition
			(base pairs)
GATA1	Forward	TACTCAGTGCACCAACTGCC	114	50°C 2 min, 95°C 10 min→ 40×[95°C 15 sec, 60°C 1 min]→ 95°C 15 sec, 60°C 30 sec, 95°C 15 sec
GATA1	Reverse	CGGTTCACCTGGTGTAGCTT	114
GR	Forward	AAGGGTTTGCTTTCACCCCA	138
GR	Reverse	AAGCGTGTTGCAATTTCCCC	138
GATA3	Forward	GAGATGGCACGGGACACTAC	102
GATA3	Reverse	CTGCAGACAGCCTTCGCTT	102
IL4	Forward	TCTTCCTGCTAGCATGTGCC	113
IL4	Reverse	GGTGCACAGAGTCTTCTGCT	113
IL5	Forward	AGCCAATGAGACTCTGAGGAT	116
IL5	Reverse	CAGTACCCCCTTGCACAGTT	116
IL13	Forward	ATGCATCCGCTCCTCAATCC	78
IL13	Reverse	AGTGAGAGCATGACCGTGG	78
GAPDH	Forward	ACCACAGTCCATGCCATCAC	452
	Reverse	TCCACCACCCTGTTGCTGTA

ISSN: 1471-2350