Table 3 Order and location of STS along the Y chromosome, sequence of the primers used and expected size of the amplified product
STS | Locus | Location | Chromosomic region | Primer sense | Primer antisense | Lenght of the amplification product (Bp*) |
|---|---|---|---|---|---|---|
sY14 | SRY | 2715341-2715810 | p11.31 | GAATATTCCCGCTCTCCGGA | GCTGGTGCTCCATTCTTGAG | 472 |
sY1301 | BV703591 | 2881785-2882279 | p11.31 | GTCTTGTTGCAGCCCATGT | CAAAGGGAGAATAGCAGGC | 495 |
Amely-3 | BV678972 | 6737830-6738427 | p11.2 | GGTGGGAGAAGGATGTTGTT | AGTCAATCCGAATGGTCAGG | 598 |
sY3218 | BV704083 | 7234129-7234319 | p11.2 | CTTTTTCGTTGGAGTCTCGC | GCAAAACCCCGTCTCTACAA | 191 |
sY1059 | G66106 | 7561835 – 7562133 | q11.223 | GATCATTCTCAAGGGGCTCA | TTGTTGTTGTTGTCATTGTGG | 299 |
TSPY | NG022996 | 9236076-9307357 | p11.2 | CGATAGGCCTCCACTTCATA | GATGACATAATGGCGGAG | 1300 |
DYZ3 | - | - | Centromérica | ACACATCACAAAGAACTATG | TGAAAACTACACAGNAAGCTG | 1100 |
sY81 | DYS271 | 12606410-12606618 | q11.1 | AGGCACTGGTCAGAATGAAG | AATGGAAAATACAGCTCCCC | 209 |
sY82 | DYS272 | 12838080-12838343 | q11.1 | ATCCTGCCCTTCTGAATCTC | CAGTGTCCACTGATGGATGA | 264 |
Y6HP35pr | DYS274 | ND | - | GGTACACACTCCATCCTGGAC | CTACAGGCTACCTTTTAGGTGG | 226 |
sY86 | DYS148 | 13117503-13117820 | q11.1 | GTGACACACAGACTATGCTTC | ACACACAGAGCGACAACCCT | 320 |
sY84 | DYS273 | 13299428-13299753 | q11.1 | AGAAGGGTCTGAAAGCAGGT | GCCTACTACCTGGAGGCTTC | 326 |
sY609 | G65840 | 13488371-13488514 | q11.21 | CATAGCCCAGAGCAATCCAT | TAGGCATAGGAAGTGGCTGG | 144 |
sY88 | DYS276 | 14113358-14113480 | q11.21 | TTGTAATCCAAATACATGGGC | CACCCAGCCATTTGTTTTAC | 123 |
sY94 | DYS279 | 14790821-14790970 | q11.21 | TCATGACAGCCAGGGTATTT | TTTGGACATAGTTTTTTGGTCC | 150 |
sY95 | DYS280 | 15053332-15053634 | q11.21 | TCCTACAGATGTCCAAAGTGC | GATGAGTGACCCCAGAATTG | 303 |
sY182 | KAL-Y | 15980790-15980914 | q11.221 | TCAGAAGTGAAACCCTGTATG | GCATGTGACTCAAAGTATAAGC | 125 |
sY97 | DYS281 | 16263633-16263736 | q11.221 | AACTTCATCAGTGTTACATCAAGG | TGTGGCATTTTGTTATGTGG | 104 |
sY151 | KAL-Y | 16020619-16020801 | q11.221 | AAATCTGTAGTCTCATATCAATCTG | TTACTTGATTTAGCAATAAAAAGG | 183 |
sY102 | DYS198 | 17080238-17080455 | q11.221 | CACTACCACATTTCTGGTTGG | CGCTGAGTCCATTCTTTGAG | 218 |
sY105 | DYS201 | 17866681-17866981 | q11.221 | AAGGGCTTCTTCTCTTGCTT | AGGGAGCTTAAACTCACCGT | 301 |
Y6D14pr | DYS205 | ND | - | GGCTAGGTGCCAGCAAGTAGATCA | GTTCTCTTCCCCTGCATCAAG | 134 |
sY117 | DS209 | 19171254-19171515 | q11.221 | GTTGGTTCCATGCTCCATAC | CAGGGAGAGAGCCTTTTACC | 262 |
Y6PHc54pr | ND | ND | - | GCAGAAGAGTTCAGC | GTGGAGTGCTGCATTAAAGG | 166 |
sY1287 | G75509 | 19491288-19491607 | q11.221 | GCAACATAGATGGACCCAGAA | ATAGCAAAGAGCCTCCCAGA | 320 |
sY1752 | BV682283 | 20229909-20230117 | q11.222 | TCAGTGTGTCACCAAGCAGG | GAGGCAAGTGGACCATCTGT | 209 |
sY127 | DYS218 | 20979804-20980078 | q11.222 | GGCTCACAAACGAAAAGAAA | CTGCAGGCAGTAATAAGGGA | 274 |
sY134 | DYS224 | 21965448-21965750 | q11.222 | GTCTGCCTCACCATAAAACG | ACCACTGCCAAAACTTTCAAA | 301 |
sY143 | DYS231 | 22387291-22387601 | q11.223 | GCAGGATGAGAAGCAGGTAG | CCGTGTGCTGGAGACTAATC | 311 |
sY1059 | G66106 | 22822646-22822944; 23252550-23252848 | q11.223 | GATCATTCTCAAGGGGCTCA | TTGTTGTTGTTGTCATTGTGG | 299 |
sY149 | DYS1 | 23725053-23725184 | q11.223 | TGTCACACTGCCCTAATCCT | TGGTCATGACAAAAGACGAA | 132 |
sY147 | DYS232 | 23882766-23882865 | q11.223 | TTTCTCGTTTGATGATCCTAG | TTAATATGAGAATGAGAACAGATGT | 100 |
Fr15-Hpr | ND | ND | - | TACCTTGGTTTTGCACCAGACGC | CACCCTCTGTATATGACCTGGC | 313 |
Y6HP52pr | DYS239 | ND | - | GGAACTGGCAGGATTAGCTTC | GCTCAGAATCTGCGATCAG | 258 |
sY1059 | G66106 | 24054410-24054708 | q11.223 | GATCATTCTCAAGGGGCTCA | TTGTTGTTGTTGTCATTGTGG | 299 |
sY157 | DYS240 | 24284845-24285134 | q11.223 | CTTAGGAAAAAGTGAAGCCG | CCTGCTGTCAGCAAGATACA | 285 |
sY1191 | G73809 | 24875620-24876004 | q11.23 | CCAGACGTTCTACCCTTTCG | GAGCCGAGATCCAGTTACCA | 385 |
sY1059 | G66106 | 26726968-26727266 | q11.223 | GATCATTCTCAAGGGGCTCA | TTGTTGTTGTTGTCATTGTGG | 299 |
sY3168 | BV704033 | 28761338-28761820 | q11.23 | GCACCACTGTACTGAAGCATG | TCCCAGCTCACATTGATGATTA | 483 |