Figure 2
From: Detailed investigations of proximal tubular function in Imerslund-Gräsbeck syndrome
Sequencing analysis of (c.1006 + 11_1008del) gAMN8-12 minigene mRNA transcript. A selected region of the trace data, obtained from RT-PCR analyses of purified mRNA from transiently transfected CHO-K1 cells with the established (c.1006 + 11_1008del) gAMN8-12 minigene construct, showed incorporation of 10 base pairs of AMN intron 9 in the spliced mRNA product. Arrow indicates point of the 70 bp deletion. Coding and intron sequence is represented by upper and lower case letters, respectively. The open reading frame (ORF) for normally spliced AMN mRNA in this region is shown below with the resulting altered reading frame for c.1006 + 11_1008del highlighted in red.