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Figure 5 | BMC Medical Genetics

Figure 5

From: Rare recessive loss-of-function methionyl-tRNA synthetase mutations presenting as a multi-organ phenotype

Figure 5

F370L and I523T MARS associate with the multisynthetase complex. A: Coomassie staining of wild-type, F370L and I523T MARS-FLAG purified fractions. MARS-FLAG is indicated (arrow). The asterisk shows a degradation product of MARS (~75 kD), which is more pronounced after purification of wild-type MARS-FLAG. However, the ratio of the 100 kD full length MARS and the partially degraded form was not established in the wild-type and MARS mutants. MARS was not immunoprecipitated from cell lysates that expressed I523T MARS-FLAG with a nonsense mutation in the FLAG tag that abolished binding to the anti-FLAG antibody (see Additional file 3: Figure S2). B: Components of the multisynthetase complex are co-purified upon immuno-isolation of wild-type, F370L or I523T MARS. Western blotting shows the presence of similar levels of MARS, KARS and RARS (upper, middle and lower panel, respectively) after immuno-isolation of MARS-FLAG from HEK293 cells.

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BMC Medical Genetics

ISSN: 1471-2350

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