Figure 2

Expression time course of the three genes within the 10q23 locus during adipogenesis in SGBS cells. mRNA and protein were extracted from SGBS cells at given time points (Days 0, 5, 9, 13, 16, 20 and 23) following introduction of the differentiation medium supplemented with Rosiglitazone. Confocal images of the SGBS cells are presented at each of these time points (A). Real-time PCR normalized for 36B4 mRNA expression (B, C) and Western blots (D) were carried out to measure either mRNA or protein levels of the gene of interest, respectively. Standard deviations were calculated based on three independent mRNA replicates. A representative Western blot result is presented.