Skip to main content
Figure 1 | BMC Medical Genetics

Figure 1

From: Expression analyses of the genes harbored by the type 2 diabetes and pediatric BMI associated locus on 10q23

Figure 1

Expression of the three genes within the 10q23 locus at both the pre-adipocyte and adipocyte stage of SGBS cell differentiation. mRNA and protein were extracted from SGBS cells either at the pre-adipocyte or adipocyte stage cultured in differentiation medium supplemented with Troglitazone for 27–49 days. Confocal image of SGBS cells in the pre-adipocyte and adipocyte states (49 days in differentiation medium) are shown (A). In (B) change in real-time PCR normalized for 36B4 mRNA expression levels of the PPARG control before and after differentiation; as expected, PPARG levels increase. In (C), change in real-time PCR normalized for 36B4 mRNA expression levels in HHEX, IDE, and KIF11 mRNA expression levels; the levels of the RARG control decrease as expected. Western blots (D) were carried out to measure either mRNA and protein levels of the genes of interest, respectively. Standard deviations were calculated based on four independent mRNA replicates. A representative Western blot result is presented.

Back to article page