Table 2 Information of Primers and Probes Used for qRT-PCR
Fragment | Primers | Probe | Amplify length |
|---|---|---|---|
SMN1 | SMN_mgb-F: 5′TGGTACATGAGTGGCTATCATACTG3′ | 5′FAM-ATGGGTTTCAGAA-MGB-NFQ | 75 bp |
|  | SMN_mgb-R: 5′ GTGAGCACCTTCCTTCTTTTT3′ |  |  |
SMN2 | SMN_mgb-F: 5′TGGTACATGAGTGGCTATCATACTG3′ | 5′FAM-ATGGGTTTTAGAA-MGB-NFQ | 75 bp |
|  | SMN_mgb-R: 5′ GTGAGCACCTTCCTTCTTTTT3′ |  |  |
GAPDH[26] | GAPDH_abs-F:5′GGGTGTGAACCATGAGAAGTATGA-3′ | 5′FAM-CAAGATCATCAGCAATGC-NFQ3′ | 73 bp |
|  | GAPDH_abs-R: 5′CTAAGCAGTTGGTGGTGCAGG-3′ |  |  |