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Table 1 Primer sequences and restriction-product characteristics

From: Polymorphisms in the genes coding for iron binding and transporting proteins are associated with disability, severity, and early progression in multiple sclerosis

Oligo name

Oligo sequence

PCR size (bp)

Restriction enzyme

Restriction products (bp)

FNP1 -8CG

    

Fw R/P

5’CCAGTTCCTTGCACTCCTG-3’

129

BstUI (60°C)

85+44 (Pol)

Rv R/P

5’CATCCTCTCTGGCGGTTG-3’ [Bio]

Sq

5’AGAGCCAGCGGGGTC-3’

HFE C282Y

    

Fw R

5’-TGGCAAGGGTAAACAGATCC-3’

387

RsaI (37°C)

247+140 (wt)

Rv R

5’-CTCAGGCACTCCTCTCAACC-3’

Fw P

5’-CGAACCTAAAGACGTATTGCC-3’

Rv P

5’-CCCAATAGATTTTCTCAGCTCCT-3’ [Bio]

Sq

5’GGAAGAGCAGAGATATACG-3’

HFE H63D

    

Fw R

5’-ACATGGTTAAGGCCTGTTGC-3’

207

BclI (50°C)

137+70 (Pol)

Rv R

5’-GCCACATCTGGCTTGAAATT-3’

Fw P

5’-CCACATCTGGCTTGAAATTCT-3’

Rv P

5’-GTTTGAAGCTTTGGGCTACG-3’ [Bio]

Sq

5’GGGCTCCACACGGCG-3’

TF P570S

    

Fw R

5’-GCTGTGCCTTGATGGTACCAGGTAA-3’

110

BstEII (60°C)

89+21 (wt)

Rv R

5’-GGACGCAAGCTTCCTTATCT-3’

Fw P

5’-GAAAAAGACTATGAGTTGCTGTGC-3’

Rv P

5’-CTGTGACCACAGCGTGATTC-3’ [Bio]

Sq

5’-TGATGGTACCAGGAA-3’

HEPC -582AG

    

Fw R

5’-ACCCTCCTGCCTTGGCCTC-3’

252

HpyCH4IV (37°C)

226+26 (Pol)

Rv R

5’-CCATTGCTTTAAGCTCTCACC-3’

Fw P

5’-ACATCTCAAGGGTCTGACACTGG-3’

Rv P

5’-GAGCAGGGCAAGCATCAGC-3’ [Bio]

Sq

5’-TCTGACACTGGGAAAAC-3’

  1. Fw and Rv indicate the forward and reverse primer, respectively; Sq indicates the sequencing primer; R and P indicate Restriction and Pyrosequencing technique, respectively; WT and Pol, indicate the wild-type (common) and polymorphic (rare) allele, respectively; [Bio], indicate the biotinylated primer.