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Table 1 Primer sequences and restriction-product characteristics

From: Polymorphisms in the genes coding for iron binding and transporting proteins are associated with disability, severity, and early progression in multiple sclerosis

Oligo name Oligo sequence PCR size (bp) Restriction enzyme Restriction products (bp)
FNP1 -8CG     
Fw R/P 5’CCAGTTCCTTGCACTCCTG-3’ 129 BstUI (60°C) 85+44 (Pol)
Rv R/P 5’CATCCTCTCTGGCGGTTG-3’ [Bio]
Sq 5’AGAGCCAGCGGGGTC-3’
HFE C282Y     
Fw R 5’-TGGCAAGGGTAAACAGATCC-3’ 387 RsaI (37°C) 247+140 (wt)
Rv R 5’-CTCAGGCACTCCTCTCAACC-3’
Fw P 5’-CGAACCTAAAGACGTATTGCC-3’
Rv P 5’-CCCAATAGATTTTCTCAGCTCCT-3’ [Bio]
Sq 5’GGAAGAGCAGAGATATACG-3’
HFE H63D     
Fw R 5’-ACATGGTTAAGGCCTGTTGC-3’ 207 BclI (50°C) 137+70 (Pol)
Rv R 5’-GCCACATCTGGCTTGAAATT-3’
Fw P 5’-CCACATCTGGCTTGAAATTCT-3’
Rv P 5’-GTTTGAAGCTTTGGGCTACG-3’ [Bio]
Sq 5’GGGCTCCACACGGCG-3’
TF P570S     
Fw R 5’-GCTGTGCCTTGATGGTACCAGGTAA-3’ 110 BstEII (60°C) 89+21 (wt)
Rv R 5’-GGACGCAAGCTTCCTTATCT-3’
Fw P 5’-GAAAAAGACTATGAGTTGCTGTGC-3’
Rv P 5’-CTGTGACCACAGCGTGATTC-3’ [Bio]
Sq 5’-TGATGGTACCAGGAA-3’
HEPC -582AG     
Fw R 5’-ACCCTCCTGCCTTGGCCTC-3’ 252 HpyCH4IV (37°C) 226+26 (Pol)
Rv R 5’-CCATTGCTTTAAGCTCTCACC-3’
Fw P 5’-ACATCTCAAGGGTCTGACACTGG-3’
Rv P 5’-GAGCAGGGCAAGCATCAGC-3’ [Bio]
Sq 5’-TCTGACACTGGGAAAAC-3’
  1. Fw and Rv indicate the forward and reverse primer, respectively; Sq indicates the sequencing primer; R and P indicate Restriction and Pyrosequencing technique, respectively; WT and Pol, indicate the wild-type (common) and polymorphic (rare) allele, respectively; [Bio], indicate the biotinylated primer.