Table 1 Primer design for fragment length analysis
SNP | Sequence (5′ → 3′) | PCR product (bp) | Annealing temperature (°C) | Restriction enzyme |
|---|---|---|---|---|
rs10934657 | TGGCAAGAGGGAGAGGAG | 139 | 55.2 | AluI |
| Â | CTTCCTCCTCTGTAAACCAGAGAGA | Â | Â | Â |
rs111472457 | CATCCGAGATGCAAGACCTAGA | 156 | 58.5 | AluI |
| Â | CCGTGAGGGATTCGGAGT | Â | Â | Â |
rs35057827 | GCATGAGGTGTTACATCACCAGCCAC | 123 | 60.9 | BtgI |
| Â | CAATCCAGTCCAACACCTGCT | Â | Â | Â |
rs61746078 | CAGCAGGATGTACAGCAGGT | 150 | 61.2 | AluI |
| Â | GTACGTATTCTGAGCCACAG | Â | Â | Â |
rs13074913 | TCTACAAGGCAGCTCGACAC | 136 | 58 | MscI |
| Â | AGGTCTTCCATCCATGGCC | Â | Â | Â |
rs61745397 | GCCAGGGACTCGGCTGGA | 154 | 60.6 | BamHI |
| Â | TCACCTCGATGGTGTACTGC | Â | Â | Â |
rs112304715 | CAGCAGGGACAGGATCTGCACT | 170 | 62.5 | SpeI |
| Â | AGCCGCTTATGAGTCTGCTCT | Â | Â | Â |
rs77832285 | TCCTGAGTGAGCTCCTGCATAT | 110 | 59 | NdeI |
| Â | GCTCGAACACCACATATTGC | Â | Â | Â |
rs2289838 | AGCCCGGAAGAAAGAATTTA | 168 | 58.2 | AluI |
| Â | TGGATGTTGCCAAAGATGATAAG | Â | Â | Â |
rs74389479 | AAGTACGAGCAACTGCCTGAG | 138 | 59.5 | AluI |
| Â | ATCAAAGAAGGTGCCCGCAA | Â | Â | Â |
rs1062749 | CTGCAAATTCGCCTTGTGGT | 162 | 55.2 | SacI |
| Â | GCTGAAGTGGCTCCTTTAGAGCT | Â | Â | Â |