Figure 7

Experimental validation of selected miRNAs. SH-SY5Y cells were transiently cotransfected with the Gaussia reporter vector harbouring the 3'UTR of ADAM10 downstream of the luciferase coding sequence together with the respective miRNAs. MiR-122, which has been described by Bai et al. [60] to target ADAM10, served as a positive control. After 48 hrs of incubation, luminescence was measured in the cell supernatant. Values obtained for control (water) treated cells were set to 100%, data represent mean ± SD of three independent experiments performed in triplicate. *** P < 0.001, ns = not significant, RLU = Relative Light Units.