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Table 2 Summary of products and their sizes after digestion with Fermentas Fast Digest® HinfI, Fermentas Fast Digest® MspI and NEB® BanII restriction enzymes

From: A novel multiplex PCR-RFLP method for simultaneous detection of the MTHFR 677 C > T, eNOS +894 G > T and - eNOS -786 T > C variants among Malaysian Malays

SNPs Band size (bp) Restriction enzyme Restriction enzyme recognized sequences
MTHFR 677 C > T   Fermentas Fast Digest® HinfI 5′-GANTC-3′
 CC-Wild type 248
 CT-Heterozygote 248, 130, 118
 TT-Homozygote 130, 118
eNOS −786 T > C   Fermentas Fast Digest® MspI 5′-CCGG -3′
 CC-Wild type 178
 TC-Heterozygote 178, 137, 41
 TT-Homozygote 137, 41
eNOS +894 G > T   NEB® BanII 5′-G(G/A)GC(T/C)C -3′
 GG-Wild type 371
 GT-Heterozygote 371, 223, 139
 TT-Homozygote 223, 139