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Table 1 Primers for MTHFR 677 C > T , eNOS +894 G > T and eNOS -786 T > C

From: A novel multiplex PCR-RFLP method for simultaneous detection of the MTHFR 677 C > T, eNOS +894 G > T and - eNOS -786 T > C variants among Malaysian Malays

Primer Sequences Length (bases) Tm* (°C) GC (%) References
677 F 5′-CTC GCC TTG AAC AGG TGG AG-3′ 20 58.3 60 Self-designed
677R 5′-CTG GAT GGG AAA GAT CCC GG-3′ 20 58 60 Self-designed
894 F 5′-GTC CCT GAG GAG GGC ATG AG-3′ 20 59.9 65 [18]
894R 5′-TCC AGC AGC ATG TTG GAC AC-3′ 20 58.2 55 [18]
786 F 5′-GCA GGT CAG CAG AGA GAC TA-3′ 20 55.9 55 [19]
786R 5′-GAC ACA GAA CTA CAA ACC CC-3′ 20 53.6 50 [19]
  1. *Tm = melting temperature of primers.