Figure 4From: Mutations in LRP5 cause primary osteoporosis without features of OI by reducing Wnt signaling activity A) Tph1 gene expression and B) 5- Htr1b gene expression in CHO cells transfected with wild type LRP5 (WT-LRP5) or mutant LRP5 and treated with Wnt3a-CM or L1-CM. Expression of the Tph1 and 5-Htr1b genes is shown as a fold change relative to the untreated control (pcDNA3.1+) and normalized to β-actin. The effects of the LRP5 mutants on the expression of these genes were examined by comparing the results for the mutants with those for WT-LRP5 using Student's t test. The statistical significance of each pair is denoted below the mutant columns; **p < 0.01 as compared to WT-LRP5. Results are expressed as mean ± SD.Back to article page