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Figure 2 | BMC Medical Genetics

Figure 2

From: A mutation at IVS1 + 5 of the von Hippel-Lindau gene resulting in intron retention in transcripts is not pathogenic in a patient with a tongue cancer?: case report

Figure 2

RT-PCR of VHL transcripts. A. Primer positions of intron and exon PCR. B. Amplification plots of real-time PCR. We performed real-time PCR for cDNA from the cancerous tongue tissue from the patient and cDNA from non-cancerous tissue of the control. GAPDH mRNA was quantified as an internal control. C. Agarose gel electrophoresis of real-time PCR products. The PCR product obtained from (B) was run on a 2% agarose gel for electrophoresis. Lane I, intron PCR (167 bp); lane E, exon PCR (144 bp); lane G, GAPDH PCR; lane G (-), GAPDH PCR using DNase-treated RNA without reverse transcription as a template; lane M, HincII digests of φx174 phage DNA.

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BMC Medical Genetics

ISSN: 1471-2350

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