RT-PCR of VHL transcripts. A. Primer positions of intron and exon PCR. B. Amplification plots of real-time PCR. We performed real-time PCR for cDNA from the cancerous tongue tissue from the patient and cDNA from non-cancerous tissue of the control. GAPDH mRNA was quantified as an internal control. C. Agarose gel electrophoresis of real-time PCR products. The PCR product obtained from (B) was run on a 2% agarose gel for electrophoresis. Lane I, intron PCR (167 bp); lane E, exon PCR (144 bp); lane G, GAPDH PCR; lane G (-), GAPDH PCR using DNase-treated RNA without reverse transcription as a template; lane M, HincII digests of φx174 phage DNA.