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Figure 1 | BMC Medical Genetics

Figure 1

From: Thymic stromal lymphopoietin gene promoter polymorphisms and expression levels in Graves’ disease and Graves’ ophthalmopathy

Figure 1

TSLP increased Th17 differentiation in human PBMC isolated from 10 control subjects and 10 GD patients. PBMC were activated by anti-CD3 and anti-CD28. Th17 differentiation was promoted by adding 10 ng/mL of TGF-β, 10 ng/mL of IL-23, 10 ng/mL of IL-6, 10 ng/mL of IL-1β, and 1 μg/mL of anti-IL4 and anti-IFNγ with (C-TSLP or P-TSLP), or without (C or P)125 ng/mL of TSLP. The concentrations of IL-17 in the culture supernatants were determined by enzyme-linked immunosorbent assay. C: control; P: patient. Values are mean ± standard deviation of 3 independent measurements. Student’s t tests have produced p values for the paired comparisons C versus P, C-TSLP versus P-TSLP, and P versus P-TSLP of 0.0381, 0.0027, and 0.0277, respectively.

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