Figure 2From: Leukotriene B4 receptor locus gene characterisation and association studies in asthma Profiling LTB4R1 and LTB4R2 variants in lung and peripheral cells. PCR was used to screen for the 5′RACE variants in various cells and tissues. A common reverse PCR primer was designed in the LTB4R1 open reading frame sequence and different forward PCR primers were designed to assay for the four variants. RT- samples did not show any amplification signals (data not shown). HASM: human airway smooth muscle; HBECu: undifferentiated human bronchial epithelial cells; HBECd: differentiated human bronchial epithelial cells; PBMC: peripheral blood mononuclear cell; PMN: polymorphonuclear neutrophil; BEAS-2B: human bronchial epithelial cell line; THP-1: human acute monocytic leukemia cell line.Back to article page