Effects of genetic variations in the Adiponectin pathway genes on the risk of colorectal cancer in the Chinese population

Table 1 Primers and PCR conditions for genotype detection of ADIPOQ and ADIPOR1

SNP	Gene	Position	Annual temperature	Primer sequence	Restriction enzyme	PCR-RFLP products(bp)	Genotype
				(5'-3')
rs266729	ADIPOQ	5′ flanking -11365 C→G	63°C	GATGTCTTGTTGAAGTTGGTGCTG TAGAAAGTTTAGGCTTGAAGTGGC	Hin6 I	176 176, 100, 76 100, 76	CC GC GG
rs822395	ADIPOQ	Intron 1 -4034 A→C	50°C	TGATCGCACCTATTAGTGGAGgAAT* TCCAGAATATGTGAAAGCCCCAGAG	Hinf I	208 208, 187, 21 187, 21	AA AC CC
rs822396	ADIPOQ	Intron 1 -3964 A→G	58°C	ATCAGAGTCCGTTCTTGGT ACTGCTTGTCACCTCCACC	Tru9 I	458 458,259,199 259,199	GG GA AA
rs2241766	ADIPOQ	Exon 2 +45 T→G	51°C	GATCAAGGTGGGCTGCAATA AGTCGTGGTTTCCTGGTCAT	Ava I	246 246,220,26 220,26	TT TG GG
rs1501299	ADIPOQ	Intron 2 +276 G→T	50°C	TCTAGGCCTTAGTTAATAATGcA* CAGGAAACCACGACTCAAG	Nsp I	250 250,230,20 230,20	AA AC CC
rs12733285	ADIPOR1	Intron 1 -1742 C→T	66°C	GTCATGCTATGCTCAACCCACAtGC* CTTCCAGCTTCCTGTTCTCCTTAG	Nsi I	209 209,183,26 183,26	CC CT TT
rs1342387	ADIPOR1	Intron 4 +5843 G→A	60°C	CACCTGGTAGTGGGATTGG GAGAAGCTGAGGCAGAGCA	Bcc I	474 474,294,180 294,180	GG AG AA

* Primers in lower case letters denote the mismatched position that is required for subsequent restriction digestion for the differentiation of the polymorphisms.

ISSN: 1471-2350