Figure 2
From: Low penetrance of retinoblastoma for p.V654L mutation of the RB1 gene
The genetic analyses of RB1 gene. (A) Exon 19 of the RB1 gene was analyzed in 30 family members and 20 unaffected individuals by high-resolution melting analysis. (B) After detection of the variant by high-resolution melting analysis, a derivative plot (-dF/dT vs temperature) of exon 19 of the RB1 gene was constructed. (C) Sequencing confirmed that the wild type cluster and the c.1960 G>T clusters were different.