Figure 4

Overlapping information of methylated- p16 by different assays. 102 oral mucosa biopsy samples were tested using three assays; red circles indicate methylated-p16 positive sample numbers as determined using the 150-bp MSP; blue circles indicate methylated-p16 positive sample numbers as determined using the 115-bp novel MethyLight assay; violet circles indicate methylated-p16 positive sample numbers as determined using the 70-bp classic MethyLight assay; the black dashed line circles represent samples without methylated-p16 detected using the three assays; the number within each open area covered by different cycles represents the exact number of samples containing methylated-p16 as detected by the corresponding assays. A: Qualitative results without the use of a cut-off point. B: Summary of the total number of samples with or without methylated-p16 as determined by each assay (left/right case no., without/with cut-off point). C: Qualitative results using a cut-off point for the two MethyLight assays (relative copy number; 0.073 for the classic MethyLight assay, and 0.0002 for the novel MethyLight assay).