Figure 4From: Mismatched single stranded antisense oligonucleotides can induce efficient dystrophin splice switching Comparison of optimised and personalised 2OMeAOs for the excision of Exon 25 in normal and patient cells. The 2OMeAOs H25A(+95+119) and patient-specific H25A(+95+A+119) were transfected into normal myoblasts (A, C and E) and MyoD adenovirus converted (c.3385 Insertion A) patient myoblasts (B, D and F) at the concentrations indicated. Nested RT-PCR was undertaken across exons 18-26 and densitometry used to quantify the levels of full length and exon 25-deleted (Δ25) amplicon in each lane as a relative measure of exon skipping efficiency. The percentage of exon 25 skipping induced by H25A(+95+119) (black line) and H25A(+95+A+119) (grey line) is presented graphically for normal (E) and patient cells (F).Back to article page