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Figure 4 | BMC Medical Genetics

Figure 4

From: Mismatched single stranded antisense oligonucleotides can induce efficient dystrophin splice switching

Figure 4

Comparison of optimised and personalised 2OMeAOs for the excision of Exon 25 in normal and patient cells. The 2OMeAOs H25A(+95+119) and patient-specific H25A(+95+A+119) were transfected into normal myoblasts (A, C and E) and MyoD adenovirus converted (c.3385 Insertion A) patient myoblasts (B, D and F) at the concentrations indicated. Nested RT-PCR was undertaken across exons 18-26 and densitometry used to quantify the levels of full length and exon 25-deleted (Δ25) amplicon in each lane as a relative measure of exon skipping efficiency. The percentage of exon 25 skipping induced by H25A(+95+119) (black line) and H25A(+95+A+119) (grey line) is presented graphically for normal (E) and patient cells (F).

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