Table 2 Primers used in PCR-restriction fragment-length polymorphism (RFLP) and gene splicing analysis

rs7216389

T/G

F:5'-GTCACATTTCCACCAGTT-3'

R:5'-CTGTAATCCCAGCACTTT-3'

563bp

Nsi I

334/229

rs12603332

A/C

F:5'-GAGTGTCTGGCATACTGGCTGG-3'

R:5'CCGAAAACTTCTGCTGCCATAGCTGGCACG-3'

214bp

BstU I

174/30

rs12936231

C/G

F:5'-TATGACATATTGTTGCTTCT-3'

R:5'-ATAGACTCACAAAGGGATTC-3'

349bp

Bsl I

191/158

rs12603332

(gene splicing)

F1:5'-GACCCTCACCAACCTCATTCAC-3'

R1:5'-CCATAATCCATCTGCTCCCAGTG-3'

129bp

F2:5'- AGGGAATGGGAAGGGCTCAC-3'

R2:5'- CGGCATGTGGCTGACAAGTG-3'

801bp