Table 1 Details of primers and probes for the Taqman Assay
From: De novo deletion in MECP2 in a monozygotic twin pair: a case report
Exon | Domain* | Forward Primer sequence | Reverse Primer sequence | Probe sequence |
|---|---|---|---|---|
3 | MBD | 5' AGCGGCGCTCCATCATC 3' | 5' TTCCGTGTCCAGCCTTCAG 3' | 5' CATGGGTCCCCGGTCAC 3' |
4 | TRD | 5' GCTCCTTGTCAAGATGCCTTTTC 3' | 5' CCATGACCTGGGTGGATGTG 3' | 5' CCCTCAGCCTTGCCC 3' |
4 | DPR | 5' GCGTCTGCAAAGAGGAGAAGAT 3' | 5' GCGGGCTGAGTCTTAGCT 3' | 5' CAGCCGTCGCTCTC 3' |