Vitamin D binding protein variants associate with asthma susceptibility in the Chinese han population

BMC Medical Genetics

Table 2 Primer sequences and PCR conditions for genotyping VDR, CYP2R1 and GC variants

Gene	Region	PCR primers(5'→3')	Sequencing primer(5'→3')	Annealing temperature(°C)	PCR product (bp)	Restriction Enzyme	RFLP products (bp)
VDR
	Exon 1	F:TGGCACCAAGGATGCCAGCT		57	364
		R:TCCTGCTCCTGTGGCTGTGA	R:GTGAGCGCCGCATGTTCC
	Exon 2	F:CCTCATGTCTTCTGTTGGAG	F:CCTCATGTCTTCTGTTGGAG	57	438
		R:TGCATCTGACCCTGGACTTC
	Exons 3,4	F: CCTATCTTGGACCTTTACCC	F: CCTATCTTGGACCTTTACCC	57	758
		R:TCCATTAGGGAGCCTTCCAC
	Exon 5	F: CTGTGGAGTCACTGTGGGAT	F: CTGTGGAGTCACTGTGGGAT	57	390
		R:GAAGTGGTGGATGAGTGATC
	Exons 6,7	F: TGTTGGTGCCCAGCAGGTGT	F: TGTTGGTGCCCAGCAGGTGT	57	680
		R:GCTACGTCTCCCTTCAGGTT
	Exon 8	F: TGCTGCCGTTGAGTGTCTGT		57	526
		R:GGTTGGACAGGAGAGAGAAT	R:ACAGGAGAGAGAATGGGCTG
CYP2R1
	Exon 1	F:GGGACCTGAGGGTATCCTGC	F:ACCTGAGGGTATCCTGCCAATG	55	409
		R:GGCGGCCATAAGTCCAACC
	Exon 2	F: AGGGACGTAACAAATCCTGG	F: AGAGCCGCACTTACCACTTG	57	412
		R: GTAGTACAGCCTGAAAGGTC
	Exon 3	F: AACAGGACCCAACCATGTAG	F: GGAGGACAATTTGGAGAAGG	57	881
		R: CATCGCAGGAGTTCCTAAAG
	Exon 4	F: TGGCTTGTTATAGGTTATCC		57	574
		R: CATTCTCTCCTGTTAGAATC	R: CATTCTCTCCTGTTAGAATC
	Exon 5	F: GTTCTGCTTGCTGAAGTGTC	F: CATAGTTCCCTCTTTCTTTG	57	415
		R: ACCAAGTTCAGGGATAAGGC
GC
	rs4588 rs7041	F:AAATAATGAGCAAATGAAAGAAGAC		55	483	Hae III	297/186
		R:CAATAACAGCAAAGAAATGAGTAGA				Sty I	305/178

ISSN: 1471-2350