Skip to main content

Table 1 Primers and probes used in end point PCR, ASPE and DH reactions.

From: Quantitative assay for the detection of the V617F variant in the Janus kinase 2 (JAK2) gene using the Luminex xMAP technology

PCR

Forward primer

5'-AGCAAGCTTTCTCACAAGCA-3'

 

Reverse primer

5'biotin-CTGACACCTAGCTGTGATCCTG-3'

DH

Wild-type probe

5'unilinker-TGGAGTATGTGTCTGTGGAGA-3'

 

Mutant probe

5'unilinker-TGGAGTATGTTTCTGTGGAGA-3'

ASPE

Forward primers

5'-AGCATTTGGTTTTAAATTATGGAGTATATT-3'

  

5'-AGCATTTGGTTTTAAATTATGGAGTATGTG-3'PO4

 

Reverse primer

5'biotin-CTGACACCTAGCTGTGATCCTG-3'

  1. The reverse primer is biotinylated at its 5' end. The probes differing by a single nucleotide (bold letter) contain a unilinker at their 5' ends for coupling to the microspheres.
Back to article page

BMC Medical Genetics

ISSN: 1471-2350

Contact us