Gene, OMIM No., nucleotide change, position and rs ID of the polymorphism | Primer sequence | Product size (bp) | Annealing temp./Restriction enzyme/allele sizes |
---|---|---|---|
ADPRT1 (*173870; 1q42.12) C>G (Phe54Leu; rs3738708) | F: 5'- TCGAGGTCAAGGTCAAGGTC -3' R: 5'- CTGTCACTCCTCCAGCTTCC -3' | 249 | 60°C/BsaX I/ C = 106,143 bp G = 249 |
ADPRT1 (*173870; 1q42.12) T>C (Val762Ala; rs1136410) | F: 5'- CCC AAA TGT CAG CAT GTA CG -3' R: 5'- AGG CCT GAC CCT GTT ACC TT -3' | 406 | 57°C/Ssi I/ T = 406 bp C = 268 bp, 138 bp |
ADPRT1 (*173870; 1q42.12) A>G (Arg940Lys; rs3219145) | F: 5'- AGA TGG TCT GGG TTT TGT GG-3' R: 5'- TGA ACC TCC AAT CAT GGT CA -3' | 399 | 57°C/Eco9I1/ A = 399 bp G = 243 bp,156 bp |
AKR1B1 (*103880; 7q35) 5' UTR G>C (rs5053) | F: 5'-ACT AGG ACC AGG CGG AAG AA -3' R: 5'- CCG TTG TTG AGC AGG AGA C-3' | 234 | 58°C/Msp I/ G = 154 bp, 80 bp C = 234 bp |
AKR1B1 (*103880; 7q35) 5' UTR, G>A (rs759853) | F: 5'-ACT AGG ACC AGG CGG AAG AA -3' R: 5'- CCG TTG TTG AGC AGG AGA C-3' | 234 | 58°C/BfaI/ G = 234 bp A = 204 bp, 20 bp |
RAGE (*600214; 6p21.3) -374T>A (rs1800624) | F: 5'- AAA ACA TGA GAA ACC CCA GA-3' R: 5'-CCC CGA TCC TAT TTA TTC CA -3' | 222 | 57°C/Alu I/ T = 222 bp A = 176 bp, 46 bp |
RAGE (*600214; 6p21.3) -429T>C (rs1800625) | F: 5'- AAA ACA TGA GAA ACC CCA GA-3' R: 5'-CCC CGA TCC TAT TTA TTC CA -3' | 222 | 57°C/Tas I/ T = 121 bp, 101 bp A = 222 bp |
RAGE (*600214; 6p21.3) Promoter, 63 bp Ins/Del polymorphism | F: 5'- AAA ACA TGA GAA ACC CCA GA-3' R: 5'-CCC CGA TCC TAT TTA TTC CA -3' | 222 | 57°C/ Ins = 222 bp Del = 159 bp |
GFPT2 (*603865; 5q34-q35 3'UTR, C>T (rs7725) | F5'-GGCTTTCTGTAGCCGTGGT-3' R5'-TACTATGGGCAAGGAGCAG-3' | 361 bp | 58°C/Tsp509I/ C = 361 bp T = 218,143 bp |
PAI-1 (*173360; 7q21.3) Promoter, PAI-1 4G/5G | F5'-CACAGAGAGAGTCTGGACACGT-3' R5'- CCCAACAGAGGACTCTTGGTC-3' | 101 bp | 62°C/Bsl I 4G = 97 bp,4 bp 5G = 74 bp, 23 bp,4 bp |