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Table 2 Polymorphisms in ADPRT1, AKR1B1, RAGE, GFPT2 and PAI-1 genes, their location, primer sequences, PCR conditions and restriction enzyme with product sizes.

From: Association analysis of ADPRT1, AKR1B1, RAGE, GFPT2 and PAI-1 gene polymorphisms with chronic renal insufficiency among Asian Indians with type-2 diabetes

Gene, OMIM No., nucleotide change, position and rs ID of the polymorphism

Primer sequence

Product size (bp)

Annealing temp./Restriction enzyme/allele sizes

ADPRT1 (*173870; 1q42.12)

C>G (Phe54Leu; rs3738708)

F: 5'- TCGAGGTCAAGGTCAAGGTC -3'

R: 5'- CTGTCACTCCTCCAGCTTCC -3'

249

60°C/BsaX I/

C = 106,143 bp

G = 249

ADPRT1 (*173870; 1q42.12)

T>C (Val762Ala; rs1136410)

F: 5'- CCC AAA TGT CAG CAT GTA CG -3'

R: 5'- AGG CCT GAC CCT GTT ACC TT -3'

406

57°C/Ssi I/

T = 406 bp

C = 268 bp, 138 bp

ADPRT1 (*173870; 1q42.12)

A>G (Arg940Lys; rs3219145)

F: 5'- AGA TGG TCT GGG TTT TGT GG-3'

R: 5'- TGA ACC TCC AAT CAT GGT CA -3'

399

57°C/Eco9I1/

A = 399 bp

G = 243 bp,156 bp

AKR1B1 (*103880; 7q35)

5' UTR G>C (rs5053)

F: 5'-ACT AGG ACC AGG CGG AAG AA -3'

R: 5'- CCG TTG TTG AGC AGG AGA C-3'

234

58°C/Msp I/

G = 154 bp, 80 bp

C = 234 bp

AKR1B1 (*103880; 7q35)

5' UTR, G>A (rs759853)

F: 5'-ACT AGG ACC AGG CGG AAG AA -3'

R: 5'- CCG TTG TTG AGC AGG AGA C-3'

234

58°C/BfaI/

G = 234 bp

A = 204 bp, 20 bp

RAGE (*600214; 6p21.3)

-374T>A (rs1800624)

F: 5'- AAA ACA TGA GAA ACC CCA GA-3'

R: 5'-CCC CGA TCC TAT TTA TTC CA -3'

222

57°C/Alu I/

T = 222 bp

A = 176 bp, 46 bp

RAGE (*600214; 6p21.3)

-429T>C (rs1800625)

F: 5'- AAA ACA TGA GAA ACC CCA GA-3'

R: 5'-CCC CGA TCC TAT TTA TTC CA -3'

222

57°C/Tas I/

T = 121 bp, 101 bp

A = 222 bp

RAGE (*600214; 6p21.3)

Promoter, 63 bp Ins/Del polymorphism

F: 5'- AAA ACA TGA GAA ACC CCA GA-3'

R: 5'-CCC CGA TCC TAT TTA TTC CA -3'

222

57°C/

Ins = 222 bp

Del = 159 bp

GFPT2 (*603865; 5q34-q35

3'UTR, C>T (rs7725)

F5'-GGCTTTCTGTAGCCGTGGT-3'

R5'-TACTATGGGCAAGGAGCAG-3'

361 bp

58°C/Tsp509I/

C = 361 bp

T = 218,143 bp

PAI-1 (*173360; 7q21.3)

Promoter, PAI-1 4G/5G

F5'-CACAGAGAGAGTCTGGACACGT-3'

R5'- CCCAACAGAGGACTCTTGGTC-3'

101 bp

62°C/Bsl I

4G = 97 bp,4 bp

5G = 74 bp, 23 bp,4 bp