The polyacrylamide gel electrophoresis analysis of ANs (A) and alleles of SCA patients (B). In figure 2A, lanes 1-5 were normal individuals. The black line indicates the upper limit of ANs. In figure 2B, lanes 1, 3, 4, and 5 were MJD patients; lane 2 was an SCA patient, but not a MJD patient. The black line indicates the place of the lower limit of expanded alleles. M: pGEM-3Zf (+) DNA-Hae III marker.