A novel DSPPmutation causes dentinogenesis imperfecta type II in a large Mongolian family

BMC Medical Genetics

Table 1 Primers used for amplification and sequencing of the DSPP gene

Exon	Forward primer sequence (5'--3')	Reverse primer sequence (5'--3')	Annealing Temperature (°C)
1	TCACCAAGTGAAGGAAGTGG	AAAGCCCAAGGTGGATTTTT	58°C
2	GATGCCCCCATAACCACACC	CTCCATGACTTCTGGGCATT	58°C
3	AAGAACCTTTTCAATAGCCAGT	TGGAGAAGTTAATGGAATGTAGCAAC	50°C
4-1	TGCAATTTGCTTTCCTTCAAG	TGTTATTGCTTCCAGCTACTTGAG	60°C
4-2	CAATGAGGATGTCGCTGTTG	TGCCATTGAAAGAAATCAGC	60°C
5-1	TTCTTTCCTCCATCCTTCCATAG	TGTCATCATTCCCATTGTTACC	60°C
5-2	CAAAAGGAGCAGAAGATGATGAC	TTGCTGCTGTCTGACTTGCT	64°C
5-3	CAAATCAGACAGTGGCAAAGGTAAAT	CACTGCTATTGCTGCTGTCGTTGCT	60°C
5-4	GACAGCAGTAATAGTAACAGCAGCG	GCTGTCGCTGCTATTGCTATCACTG	60°C
5-5	GCAGTGACAGCAACGAAAGCAGCAAT	GTTGTTACCGTTACCAGACTTGCTC	55°C
5-6	TGACAGCACATCTGACAGCAAT	TCCCCCAGTTGTTTTTGTTT	55°C

ISSN: 1471-2350