From: Toll-like receptor 2 gene polymorphisms, pulmonary tuberculosis, and natural killer cell counts
Polymorphism (DNA position relative to ATG) | rs number | Primers and conditions for PCR |
---|---|---|
-100 (GT)n | rs34692294 | Forward: ionhs5'-GCATTGCTGAATGTATCAGGGA-3' Reverse: 5'-CCACAAAGTATGTGCCATGGTCCAGTGCTTC-3' Condition: 95°C, 3 min; (95°C, 30 sec; 55°C, 30 sec; 72°C, 1 min) × 35 cycles; 60°C, 60 min |
-16934 A>T | rs4696480 | Forward: 5'-TGGTTCTGGAGTCTGGGAAG-3' Reverse: 5'-ACAGAACGGTCTCCAAGTAG-3' Condition: 94°C, 5 min; (94.1°C, 1 min; 59.3°C, 1 min; 72.2°C, 2 min)× 35 cycles; 72°C, 10 min. |
-15607 A>G | rs1898830 | Forward: 5'-GCAGCTGAAATCACAGAGCA Reverse: 5'-AGGATAATGGCCTCCTGCT Condition: 94°C, 5 min; (94°C,1 min; 67.1°C,40 sec; 72°C,2 min) × 30 cycle; 72°C, 10 min |
-196 to -174 Ins>del | not available | Forward: 5'-cacggaggcagcgagaaa Reverse: 5'-ctgggccgtgcaaagaag Condition: 94°C, 5 min; (94°C,1 min; 64.5°C,1 min; 72°C,2 min) × 35 cycle; 72°C, 10 min |
1350 T>C (S450S) | rs3804100 | Forward: 5'-AACCGGAGAGACTTTGCTCA Reverse: 5'-AGTTATTGCCACCAGCTTCC Condition: 94°C, 5 min; (94°C,40 sec; 62°C,40 sec; 72°C,1 min) × 30 cycle; 72°C, 10 min |