Figure 3From: Suggestive evidence of associations between liver X receptor β polymorphisms with type 2 diabetes mellitus and obesity in three cohort studies: HUNT2 (Norway), MONICA (France) and HELENA (Europe) Functional analyses of the rs28514894 SNP in luciferase reporter gene transfections in HepG2 (A) and JEG3 (B) cells. HepG2 and JEG3 cells were transiently co-transfected with either the empty basic-pGL3 (O) or the pGL3-LXRβ*T allele or the pGL3-LXRβ*C allele vector with or without an HNF4α or NF1s expression vector. Activity was expressed as fold activity compared with the pGL3-LXRβ*T vector and the empty basic-pGL3 vector. White and black bars represent activities obtained without or with HNF4α or NF1s, respectively. Each bar represents the means ± SEM of at least 3 independent experiments. Firefly luciferase activities were normalized against renilla luciferase activities. Data were compared using the Kruskal-Wallis non-parametric test. *p < 0.05, ** p < 0.01.Back to article page