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Figure 2 | BMC Medical Genetics

Figure 2

From: Suggestive evidence of associations between liver X receptor β polymorphisms with type 2 diabetes mellitus and obesity in three cohort studies: HUNT2 (Norway), MONICA (France) and HELENA (Europe)

Figure 2

EMSA experiments showed no difference in TF binding between the rs28514894 SNP alleles, as exemplified by NF1C binding. EMSA of nuclear extracts (2 μg) isolated from JEG3 cells transfected with the NF1C expression vector revealed a strong specific complex with the LXRβ oligonucleotides encompassing the rs28514894 SNP with both alleles (T or C). The competition experiments were performed using a 10-fold excess of unlabeled oligonucleotides (indicated by *). The upper arrow indicates the supershift with anti-HA-mouse antibody binding to the NF1C protein-oligonucleotide complex and the lower arrow indicates the NF1C protein-oligonucleotide complexes.

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BMC Medical Genetics

ISSN: 1471-2350

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