No association of the polymorphisms of the frizzled-related protein gene with peak bone mineral density in Chinese nuclear families

Table 1 Information and the primer and probe sequences for the studied SNPs in the FRZB gene

dbSNP	Polymorphism	Domain	primer sequence 5'-3'	TaqMan probe sequence
rs6433993	A/G	intron 1	F: CTGCTGAAATTAACATACCTGACCTGGATTT AAATATATACCAGTTCAGTGTGATAGGTC R: CAAAATTTGTAAATGATAAGCATCCTA
rs409238	A/G	intron 2	F: TCTGGAGCACCTTTGGAACAG R: GGGAACATTAGTGTAAGTCAGATGCT	VIC-CGCCAAGAACAGGTT FAM-CGCCAAGAGCAGGTT
rs288324	A/G	intron 5	F: CTTGAAATGCATCTCCCTTTTGACA R: GTAAGGAGAAACTACCCTCCAGTAAGT	VIC-TCTTCTCCCCTTTAGTAGAT FAM-TCTCCCCTTCAGTAGAT
rs4666865	A/G	3'near gene	F: TGGTTCTACTAATAGCACACATGTAAATGG R: CTTAGAGCCTGTGCCAATTACTTG	VIC-CAGAGAATGAAACTTT FAM-CAGAGAATGAGACTTT

The rs6433993 was genotyped by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) and did not use a TaqMan probe

ISSN: 1471-2350